Prof shehabeldin Ismael
Professor of Endodontic Department Director of the Center of Innovative Dental Sciences (CIDS), British University in Egypt
Title: Cytocompatibility of root canal sealers with different compositions and setting mechanisms on human periodontal ligament stem cells.
Biography
Biography: Prof shehabeldin Ismael
Abstract
Statement of the problem: Root canal sealers are responsible for the principal functions of the final root filling; which are sealing-off of the root canal system, entombment of the remaining bacteria and filling of irregularities in the prepared root canal system. An ideal sealer should be biocompatible, bacteriostatic, does not shrink on setting, insoluble in oral fluids and in case of extrusion from canal, it should be biocompatible. However, all root canal sealers exhibit some form of toxicity irrespective of their type. Therefore, cytotoxicity of sealers remains an issue even though newer sealers have been introduced due to their high biocompatibility. The purpose of this study was to assess the cytocompatibility of two root canal sealers; GuttaFlow 2 based on polydimethylsiloxane and incorporating gutta percha powder, and EndoSequence which is a pre-mixed paste based on calcium-phosphate-silicate.
Methodology: Human periodontal ligament stem cells (hPDLSCs) were isolated, cultured and their mesenchymal phenotype characterized by flow cytometry. The invitro potential for multilineage differentiation of the hPDLSCs was analyzed in osteogenic, adipogenic and chondrogenic media. The proliferation rate of the hPDLSCs growing in the presence of the endodontic sealer eluates was evaluated using the MTT assay, in which 3 dilutions were prepared and cell viability was analyzed after 1, 3 and 7 days of culture growth.
Results: Both GuttaFlow 2 and EndoSequence BC supported stem cells survival and showed comparable non-significant excellent proliferation rates (p>0.05).
Conclusion and significance: GuttaFlow 2 root canal sealer and EndoSequence BC Sealer were biocompatible with hPDLSCs and can be used during root canal obturation.